Journal: Bio-protocol
Article Title: Optogenetic Approach for Investigating Descending Control of Nociception in Ex Vivo Spinal Cord Preparation
doi: 10.21769/BioProtoc.5483
Figure Lengend Snippet: Left: Overview of the experimental setup showing perfusion inflow and outflow tubing, a low-magnification objective, a high-magnification water-immersion objective, and both white and infrared (IR)-LEDs mounted on a micromanipulator. The LEDs should be connected to an adjusted power supply unit and positioned so that their beams strike the plane of the experimental chamber at a 10–20° angle. Note that the LEDs must remain above the bath solution and should not be submerged. Right: Overview of spinal cord preparation in the experimental chamber. The ex vivo spinal cord preparation is affixed to a metal plate at an approximately 45° angle so that the dorsal horn is on top. The spared dorsal root is connected to a suction electrode and is slightly pulled to reveal the underlying dorsal horn. Modified from [17].
Article Snippet: Patch clamp micromanipulator (Scientifica, model: PatchStar) 5.
Techniques: Ex Vivo, Modification